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Streak-For-Isolation Technique

Navigation » List of Schools » Prince George Community College » Biology » Bio 2010 – Microbiology » Summer 2023 » Streak-For-Isolation Technique

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Below are the questions for the exam with the choices of answers:

Question #1

After making and incubating your Streak-for-Isolation plate a student notices that they have confluent growth in EVERY sector/quadrant. Which of the following most likely explains what happened?

A they left the top of the Petri open too long during making the streak

B the tryptic soy agar plate was contaminated prior to making the streak

C they returned the flame loop to the broth between every sector/quadrant

D the bacteria spread through each sector during incubation

Question #2

A student in our class makes their first Streak-for-Isolation plate and has it returned to them after incubation. Looking at it, you notice that there is bacterial growth in the first two quadrants/sectors, but not in the last two quadrants/sectors. Which of the following is the most likely explanation for this observation?

A they forgot to flame their loop before making the next sector/quadrant

B they did not incubate the agar plate long enough

C they did not incubate the agar plate at the ideal temperature

D they did not make contact in the second sector/quadrant while making the third sector/quadrant.

Question #3

Where should you label your Petri plate with information about the culture?

A on the inside of the lid.

B on the top of the lid.

C on the bottom of the plate.

D on the side of the bottom (agar-containing) portion of the plate.

Question #4

What does the streak technique enable us to achieve?

A direct spread and dilution of bacteria from a single colony

B visualization of virus plaques

C isolation of bacterial colonies

D development of antibacterial microbes

Question #5

After each streak on the plate with the inoculating loop, what is done to the loop?

A flamed until loop glows

B discarded

C re-introduced to stock culture

D wiped with disinfectant

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Manush

Fresh Graduate

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