Question
When selecting a photometric technique that is more sensitive to a low concentration of analyte, a laboratory director compares absorbance measuring devices against those that measure fluorescence intensity. Which technique is considered more sensitive in its measurements and why?
A Absorbance measurements are more sensitive because the flame can break down complex compounds into elements that are specifically measured.
B Absorbance measurements are more sensitive because of the ability of the monochromator to isolate very specific parts of the spectrum.
C Fluorescence intensity measurements are more sensitive because the fluorophores used are very specific to the analytes they bind to and will bind to small analytes easily.
D Fluorescence intensity measurements are more sensitive because of the use of intense light sources, signal filtering, and sensitive light emission photometers.
This question is taken from Chem 40005 – Clinical Chemistry » Summer 2021 » Module 2 Quiz
