From the following which is the best description for fluorescence microscopy

A  uses a light microscope with an optical component to take advantage of the different refractive indices of light passing through different regions of the cell

B  scans the specimen with a focused laser beam to obtain a series of two-dimensional optical sections, which can be used to reconstruct an image of the specimen in three dimensions. The laser excites a fluorescent dye molecule, and the emitted light from each illuminated point is captured through a pinhole and recorded by a detector.

C  has the ability to resolve cellular components as small as 2 nm.

D  employs a light microscope and requires that samples be fixed and stained in order to reveal cellular details

E  requires coating the sample with a thin layer of a heavy metal to produce three dimensional images of the surface of a sample

F  requires the use of two sets of filters. The first filter narrows the wavelength range that reaches the specimen and the second blocks out all wavelengths that pass back up to the eyepiece except for those emitted by the dye in the sample.