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Fresh Grad Lands Job as Real Estate Agent With Help from Professional Writers

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Lab Exam 1

Navigation   » List of Schools  »  Prince George Community College  »  Biology  »  Bio 2010 – Microbiology  »  Spring 2023  »  Lab Exam 1

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Below are the questions for the exam with the choices of answers:

Question #1
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A  Pseudomonas fluorescens
B  Bacillus subtilis
C  Escherichia coli
D  Serratia marcescens
Question #2
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A  It carves a path in the agar that promotes isolation
B  it reduces the number of bacteria as we move them from sector I to sector IV
C  it is good practice for other lab related techniques
D  it increases the number of bacteria as we move them from sector I to sector IV
Question #3
Which of the following is a positive Starch Agar final product?
A  nucleotides
B  amino acids
C  fructose
D  glucose
Question #4
Which of the following sectors on a plate prepared for streaking for isolation should have isolated colony growth?
A  II
B  IV
C  III
D  I
Question #5
Which of the following exoenzymes does the Spirit Blue Agar test for?
A  Amylase
B  Caseinase
C  Gelatinase
D  Lipase
Question #6
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A  Bacillus subtilis
B  Neisseria sicca
C  Escherichia coli
D  Staphylococcus epidermids
Question #7
From our microbial control lab experiments, which of the following disinfectant/antiseptic is classified as a Quaternary Ammonium Compound (Quat)?
A  zephiran chloride
B  bleach
C  lysol
D  alcohol
Question #8
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A  none of these
B  safranin
C  carbolfuchsin
D  crystal violet
E  acid alcohol
Question #9
Suppose we conduct a hand washing experiment. From our 0.2 ml sample. From this sample we counted 220 cells. How many colony forming units (CFU’s) are there per Liter?
A  1.1 X E4
B  1.1 X E5
C  1.1 X E2
D  1.1 X E6
E  1.1 X E3
Question #10
Which of the following is the Ziehl-Neelsen acid fast secondary stain?
A  crystal violet
B  Loefflerâ s methylene blue
C  safranin
D  malachite green
E  none of these
Question #11
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A  acid alcohol
B  safranin
C  crystal violet
D  none of these
E  carbolfuchsin
Question #12
Which of the following is the substrate found in Skim Milk Agar?
A  gelatin
B  casein
C  triglyceride
D  starch
Question #13
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A  Staphylococcus aureus
B  Neisseria sicca
C  Mycobacterium smegmatis
D  Escherichia coli
E  Pseudomonas fluorescens
Question #14
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A  Petri plates covered with paper.
B  Petri plates covered with aluminum foil.
C  Petri plates with plastic lids.
D  Petri plates with glass lids.
E  Open Petri plates.
Question #15
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A  crystal violet
B  peptydoglycan
C  mycolic acid
D  lactic acid
Question #16
Which of the following is the best definition of contamination?
A  the presence of bacteria
B  site of infection
C  the presence of pathogens
D  an accidental spill
Question #17
Which of the following is the Gram’s secondary stain?
A  Crystal violet
B  Loeffler’s methylene blue
C  Carbolfuchsin
D  Acetone- Alcohol
E  Safranin
Question #18
Which of the following is the Gram’s destain or bleaching reagent?
A  Loeffler’s methylene blue
B  Safranin
C  Acetone- Alcohol
D  Crystal violet
E  Carbolfuchsin
Question #19
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A  10oC
B  20oC
C  37oC
D  25oC
Question #20
Which of the following terms regarding the presence of microorganisms means “everywhere”?
A  sporadic
B  claryvoyant
C  ubiquitous
D  tropical
Question #21
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A  agar cannot be re-melted
B  the water temperature was not cool enough
C  cooling the agar it destroyed it
D  the water temperature was not hot enough
E  none of these
F  more than one of the above is correct
Question #22
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A  pipettor
B  flame loop
C  finger
D  flame wand
Question #23
How should you sterilize materials that will make contact with your culture media?
A  none of these
B  more than one of these are correct
C  by using the Bunsen burner and flaming them
D  wiping it down with a tissue
E  wash them in the sink
F  you don’t need to sterilize materials that will make contact with your experiment
Question #24
Which of the following choices is the BEST definition of depth-of-field?
A  the image that one sees when looking through the oculars
B  the ability to focus above or below a stained specimen
C  the ability to distinguish two small objects as separate
D  the distance between the end of the microscope objective lens and the specimen slide
Question #25
What is the purpose of using oil on the oil immersion lens?
A  to increase contrast of the specimen
B  to prevent light refraction away from the objective lens
C  to lubricate the sample slide and the objective
D  to clean the microscope stage elevator
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Manush
Fresh Graduate
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