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Fresh Grad Lands Job as Real Estate Agent With Help from Professional Writers

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Lab Exam 1

Navigation   » List of Schools  »  Prince George Community College  »  Biology  »  Bio 2010 – Microbiology  »  Spring 2023  »  Lab Exam 1

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Below are the questions for the exam with the choices of answers:

Question #1
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A  Serratia marcescens
B  Escherichia coli
C  Pseudomonas fluorescens
D  Bacillus subtilis
Question #2
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A  it increases the number of bacteria as we move them from sector I to sector IV
B  it is good practice for other lab related techniques
C  It carves a path in the agar that promotes isolation
D  it reduces the number of bacteria as we move them from sector I to sector IV
Question #3
Which of the following is a positive Starch Agar final product?
A  nucleotides
B  glucose
C  fructose
D  amino acids
Question #4
Which of the following sectors on a plate prepared for streaking for isolation should have isolated colony growth?
A  II
B  III
C  I
D  IV
Question #5
Which of the following exoenzymes does the Spirit Blue Agar test for?
A  Gelatinase
B  Lipase
C  Caseinase
D  Amylase
Question #6
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A  Escherichia coli
B  Staphylococcus epidermids
C  Bacillus subtilis
D  Neisseria sicca
Question #7
From our microbial control lab experiments, which of the following disinfectant/antiseptic is classified as a Quaternary Ammonium Compound (Quat)?
A  lysol
B  alcohol
C  bleach
D  zephiran chloride
Question #8
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A  acid alcohol
B  safranin
C  none of these
D  carbolfuchsin
E  crystal violet
Question #9
Suppose we conduct a hand washing experiment. From our 0.2 ml sample. From this sample we counted 220 cells. How many colony forming units (CFU’s) are there per Liter?
A  1.1 X E4
B  1.1 X E2
C  1.1 X E5
D  1.1 X E3
E  1.1 X E6
Question #10
Which of the following is the Ziehl-Neelsen acid fast secondary stain?
A  none of these
B  crystal violet
C  Loefflerâ s methylene blue
D  malachite green
E  safranin
Question #11
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A  acid alcohol
B  safranin
C  none of these
D  carbolfuchsin
E  crystal violet
Question #12
Which of the following is the substrate found in Skim Milk Agar?
A  triglyceride
B  casein
C  starch
D  gelatin
Question #13
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A  Staphylococcus aureus
B  Neisseria sicca
C  Escherichia coli
D  Pseudomonas fluorescens
E  Mycobacterium smegmatis
Question #14
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A  Petri plates with plastic lids.
B  Petri plates covered with aluminum foil.
C  Petri plates covered with paper.
D  Open Petri plates.
E  Petri plates with glass lids.
Question #15
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A  crystal violet
B  lactic acid
C  peptydoglycan
D  mycolic acid
Question #16
Which of the following is the best definition of contamination?
A  site of infection
B  an accidental spill
C  the presence of bacteria
D  the presence of pathogens
Question #17
Which of the following is the Gram’s secondary stain?
A  Safranin
B  Loeffler’s methylene blue
C  Carbolfuchsin
D  Crystal violet
E  Acetone- Alcohol
Question #18
Which of the following is the Gram’s destain or bleaching reagent?
A  Safranin
B  Crystal violet
C  Loeffler’s methylene blue
D  Acetone- Alcohol
E  Carbolfuchsin
Question #19
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A  10oC
B  25oC
C  20oC
D  37oC
Question #20
Which of the following terms regarding the presence of microorganisms means “everywhere”?
A  tropical
B  ubiquitous
C  claryvoyant
D  sporadic
Question #21
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A  none of these
B  the water temperature was not cool enough
C  cooling the agar it destroyed it
D  the water temperature was not hot enough
E  agar cannot be re-melted
F  more than one of the above is correct
Question #22
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A  flame loop
B  flame wand
C  pipettor
D  finger
Question #23
How should you sterilize materials that will make contact with your culture media?
A  none of these
B  wash them in the sink
C  wiping it down with a tissue
D  by using the Bunsen burner and flaming them
E  you don’t need to sterilize materials that will make contact with your experiment
F  more than one of these are correct
Question #24
Which of the following choices is the BEST definition of depth-of-field?
A  the image that one sees when looking through the oculars
B  the distance between the end of the microscope objective lens and the specimen slide
C  the ability to distinguish two small objects as separate
D  the ability to focus above or below a stained specimen
Question #25
What is the purpose of using oil on the oil immersion lens?
A  to lubricate the sample slide and the objective
B  to clean the microscope stage elevator
C  to prevent light refraction away from the objective lens
D  to increase contrast of the specimen
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Manush
Fresh Graduate
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