Exam 3

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Question #1

The restriction enzyme EcoRI creates sticky ends after cutting.

A TRUE

B FALSE

Question #2

A frameshift mutation will not occur if only one nucleotide is added/deleted to the DNA sequence.

A FALSE

B TRUE

Question #3

DNA is positive which allows it to travel down a gel in gel electrophoresis when a current is applied.

A TRUE

B FALSE

Question #4

Heat shock and electroporation are ways to force transformation.

A TRUE

B FALSE

Question #5

Transduction is limited by the DNA carrying capacity of the viral capsid.

A TRUE

B FALSE

Question #6

RecA is a DNA repair enzyme that facilitates the recombination of DNA.

A FALSE

B TRUE

Question #7

Biological fitness has to do with how strong an organism is versus those in its environment.

A FALSE

B TRUE

Question #8

MutL is an enzyme that links MutH and MutS in methyl mismatch repair.

A TRUE

B FALSE

Question #9

The protein that lacZ transcribes is responsible for digesting lactose into glucose and galactose.

A FALSE

B TRUE

Question #10

A bacterial cell’s genome is continuously expressed because it requires all of the proteins it encodes for at all times.

A TRUE

B FALSE

Question #11

Which of the following techniques would I use in order to analyze and visualize the presence of target proteins in a cell/tissue?

A PCR

B Immunofluorescence

C DNA Hybridization

D DNA sequencing

E None of the above

Question #12

Which of the following techniques would I use in order to analyze and visualize the presence of a target sequence of DNA in a cell/tissue?

A PCR

B Immunofluorescence

C DNA Hybridization

D DNA sequencing

E None of the above

Question #13

Which of the following is necessary in a gel electrophoresis in order to determine the size of all of the DNA fragments?

A Negative control

B DNA ladder

C Positive control

D Ruler

E Loading dye

Question #14

Which of the following restriction enzymes produce blunt ends?

A HindIII

B BamHI

C EcoRI

D HaeIII

E None of the above

Question #15

The link between Wolbachia and nematodes can be defined by:

A Environmental competition

B Parasitism

C Endosymbiosis

D Predator/prey relationship

E None of the above

Question #16

Which of the following is a mechanism of evolution?

A Reductive evolution

B Genetic drift

C Random mutation

D Natural selection

E All of the above

Question #17

Transposition is a crucial gene transfer mechanism. However it can also result in which of the following?

A Nonsense mutation

B Frameshift mutation

C Increased competency

D Cell lysis

E None of the above

Question #18

Single-strand recombination produces which of the following after replication and cell division?

A Two daughter cells with recombinant genomes

B Two daughter cells with normal genomes

C The cell would fail to replicate

D One daughter cell with a recombinant genome and one with a normal genome

E None of the above

Question #19

Which of the following horizontal gene transfer mechanisms involve the transfer of genes from a plasmid onto a bacterial host genome?

A Transduction

B Transposition

C Transformation

D Conjugation

E All of the above

Question #20

A prokaryote protects its genomic DNA by doing which of the following?

A Creating a nucleus

B Adding methyl groups to sites on the DNA

C Adding phosphate groups to sites on the DNA

D Keeping the DNA away from restriction enzymes

E None of the above

Question #21

Conjugation involves the transfer of the _____ of the plasmid DNA strand. ______ cell(s) end up with circularized plasmid.

A 3’ end ; The recipient

B 5’ end ; The recipient

C 5’ end ; Both

D 3’ end ; The donor

E 3’ end ; Both

Question #22

Which of the following horizontal gene transfer mechanisms require cell to cell contact to initiate?

A Transduction

B Transformation

C Transposition

D Conjugation

E All of the above

Question #23

Which of the following horizontal gene transfer mechanisms involve the use of bacteriophages?

A Transduction

B Transposition

C Conjugation

D Transformation

E All of the above

Question #24

A heritable mutation has to have which of the following characteristics?

A The mutation is repaired before replication and the mutation must not alter the amino acid sequence

B The mutation is not repaired before replication and the base sequence must change

C The mutation is not repaired before replication and the mutation must not alter the amino acid sequence

D The mutation is repaired before replication and the base sequence must change

Question #25

In methyl mismatch repair, which of the following enzymes identify the nearest methylation site?

A Mut S

B Mut H

C Mut L

D Exonuclease

E DNA Polymerase III

Question #26

In methyl mismatch repair, which of the following enzymes identify the site of the mutation?

A Mut H

B DNA Polymerase III

C Mut L

D Mut S

E Exonuclease

Question #27

Which of the following enzymes are involved in cleaving and removing the phosphodiester backbone in base excision repair?

A DNA polymerase I and DNA ligase

B Endonuclease and DNA ligase

C DNA polymerase I and Endonuclease

D DNA glycosylase and DNA ligase

Question #28

Which of the following enzymes extract the damaged base in base excision repair?

A DNA glycosylase

B DNA polymerase I

C Endonuclease

D DNA ligase

E None of the above

Question #29

You observe a mutation in a gene in which the amino acid sequence now has an early STOP codon. This is known as which of the following?

A Inversion

B Insertion

C Silent mutation

D Missense mutation

E Nonsense mutation

Question #30

You observe a mutation in a gene in which the amino acid sequence is not affected. This is known as which of the following?

A Nonsense mutation

B Silent mutation

C Inversion

D Insertion

E Missense mutation

Question #31

A repressor protein binds to which of the following sequences?

A Inducer

B Coding region

C Operator

D Promoter

E None of the above

Question #32

You observe a lac operon which has a faulty lacI gene that translates to an inactive protein. Which of the following would occur as a result:

A An inability to digest lactose into glucose and galactose

B Constant expression of the lac operon genes

C An inability to transport lactose into the cell

D Constant repression of the lac operon genes

E None of the above

Question #33

In the lactose operon which of the following is responsible for transporting lactose into the cell?

A Galactose

B B-Galactosidase

C LacI

D Lactose permease

E Allolactose

Question #34

In the lactose operon which of the following causes the repressor protein to fall off when it binds to it?

A LacI

B B-Galactosidase

C Lactose permease

D Allolactose

E Galactose

Question #35

The DtxR protein requires the binding of _______ which then _________ the expression of the dtx toxin.

A Fe2+ ; Up-regulates

B Fe2+ ; Down-regulates

C Cl- ; Up-regulates

D H+ ; Down-regulates

E H+ ; Up-regulates

Question #36

Which of the following levels of genetic regulation involves the binding of cofactors/coenzymes onto a protein in order to make it functional?

A Translational control

B Post Translational Control

C Control of Transcription

D Changing the DNA sequence

E None of the above

Question #37

Which of the following levels of genetic regulation are the most drastic and difficult to reverse?

A Control of Transcription

B Changing the DNA sequence

C Translational control

D Post Translational Control

E None of the above

Question #38

A regulatory protein binding to a regulatory DNA sequence is an example of which of the following levels of gene expression?

A Changing the DNA sequence

B Control of Transcription

C Post Translational Control

D Translational control

E None of the above

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