Exam 3

Navigation » List of Schools » Glendale Community College » Biology » Biology 112 – Microbiology » Fall 2021 » Exam 3

Need help with your exam preparation?

Existing Quiz Clients Login here

Below are the questions for the exam with the choices of answers:

Try The Exam

Question #1

The restriction enzyme EcoRI creates sticky ends after cutting.

A FALSE

B TRUE

Question #2

A frameshift mutation will not occur if only one nucleotide is added/deleted to the DNA sequence.

A TRUE

B FALSE

Question #3

DNA is positive which allows it to travel down a gel in gel electrophoresis when a current is applied.

A FALSE

B TRUE

Question #4

Heat shock and electroporation are ways to force transformation.

A TRUE

B FALSE

Question #5

Transduction is limited by the DNA carrying capacity of the viral capsid.

A TRUE

B FALSE

Question #6

RecA is a DNA repair enzyme that facilitates the recombination of DNA.

A TRUE

B FALSE

Question #7

Biological fitness has to do with how strong an organism is versus those in its environment.

A FALSE

B TRUE

Question #8

MutL is an enzyme that links MutH and MutS in methyl mismatch repair.

A TRUE

B FALSE

Question #9

The protein that lacZ transcribes is responsible for digesting lactose into glucose and galactose.

A FALSE

B TRUE

Question #10

A bacterial cell’s genome is continuously expressed because it requires all of the proteins it encodes for at all times.

A FALSE

B TRUE

Question #11

Which of the following techniques would I use in order to analyze and visualize the presence of target proteins in a cell/tissue?

A DNA Hybridization

B DNA sequencing

C Immunofluorescence

D PCR

E None of the above

Question #12

Which of the following techniques would I use in order to analyze and visualize the presence of a target sequence of DNA in a cell/tissue?

A DNA sequencing

B Immunofluorescence

C PCR

D DNA Hybridization

E None of the above

Question #13

Which of the following is necessary in a gel electrophoresis in order to determine the size of all of the DNA fragments?

A Positive control

B Loading dye

C DNA ladder

D Ruler

E Negative control

Question #14

Which of the following restriction enzymes produce blunt ends?

A EcoRI

B BamHI

C HaeIII

D HindIII

E None of the above

Question #15

The link between Wolbachia and nematodes can be defined by:

A Environmental competition

B Endosymbiosis

C Predator/prey relationship

D Parasitism

E None of the above

Question #16

Which of the following is a mechanism of evolution?

A Genetic drift

B Reductive evolution

C Random mutation

D Natural selection

E All of the above

Question #17

Transposition is a crucial gene transfer mechanism. However it can also result in which of the following?

A Frameshift mutation

B Increased competency

C Cell lysis

D Nonsense mutation

E None of the above

Question #18

Single-strand recombination produces which of the following after replication and cell division?

A The cell would fail to replicate

B One daughter cell with a recombinant genome and one with a normal genome

C Two daughter cells with recombinant genomes

D Two daughter cells with normal genomes

E None of the above

Question #19

Which of the following horizontal gene transfer mechanisms involve the transfer of genes from a plasmid onto a bacterial host genome?

A Transduction

B Conjugation

C Transposition

D Transformation

E All of the above

Question #20

A prokaryote protects its genomic DNA by doing which of the following?

A Adding phosphate groups to sites on the DNA

B Creating a nucleus

C Adding methyl groups to sites on the DNA

D Keeping the DNA away from restriction enzymes

E None of the above

Question #21

Conjugation involves the transfer of the _____ of the plasmid DNA strand. ______ cell(s) end up with circularized plasmid.

A 3’ end ; The donor

B 3’ end ; Both

C 5’ end ; Both

D 3’ end ; The recipient

E 5’ end ; The recipient

Question #22

Which of the following horizontal gene transfer mechanisms require cell to cell contact to initiate?

A Conjugation

B Transformation

C Transposition

D Transduction

E All of the above

Question #23

Which of the following horizontal gene transfer mechanisms involve the use of bacteriophages?

A Transduction

B Conjugation

C Transformation

D Transposition

E All of the above

Question #24

A heritable mutation has to have which of the following characteristics?

A The mutation is not repaired before replication and the base sequence must change

B The mutation is repaired before replication and the mutation must not alter the amino acid sequence

C The mutation is repaired before replication and the base sequence must change

D The mutation is not repaired before replication and the mutation must not alter the amino acid sequence

Question #25

In methyl mismatch repair, which of the following enzymes identify the nearest methylation site?

A DNA Polymerase III

B Mut S

C Exonuclease

D Mut L

E Mut H

Question #26

In methyl mismatch repair, which of the following enzymes identify the site of the mutation?

A Mut L

B Mut H

C Mut S

D Exonuclease

E DNA Polymerase III

Question #27

Which of the following enzymes are involved in cleaving and removing the phosphodiester backbone in base excision repair?

A DNA polymerase I and Endonuclease

B DNA glycosylase and DNA ligase

C Endonuclease and DNA ligase

D DNA polymerase I and DNA ligase

Question #28

Which of the following enzymes extract the damaged base in base excision repair?

A DNA glycosylase

B DNA polymerase I

C Endonuclease

D DNA ligase

E None of the above

Question #29

You observe a mutation in a gene in which the amino acid sequence now has an early STOP codon. This is known as which of the following?

A Nonsense mutation

B Inversion

C Silent mutation

D Insertion

E Missense mutation

Question #30

You observe a mutation in a gene in which the amino acid sequence is not affected. This is known as which of the following?

A Nonsense mutation

B Silent mutation

C Missense mutation

D Insertion

E Inversion

Question #31

A repressor protein binds to which of the following sequences?

A Inducer

B Operator

C Promoter

D Coding region

E None of the above

Question #32

You observe a lac operon which has a faulty lacI gene that translates to an inactive protein. Which of the following would occur as a result:

A Constant expression of the lac operon genes

B Constant repression of the lac operon genes

C An inability to transport lactose into the cell

D An inability to digest lactose into glucose and galactose

E None of the above

Question #33

In the lactose operon which of the following is responsible for transporting lactose into the cell?

A LacI

B Galactose

C B-Galactosidase

D Lactose permease

E Allolactose

Question #34

In the lactose operon which of the following causes the repressor protein to fall off when it binds to it?

A Allolactose

B LacI

C Galactose

D B-Galactosidase

E Lactose permease

Question #35

The DtxR protein requires the binding of _______ which then _________ the expression of the dtx toxin.

A Fe2+ ; Down-regulates

B H+ ; Down-regulates

C H+ ; Up-regulates

D Fe2+ ; Up-regulates

E Cl- ; Up-regulates

Question #36

Which of the following levels of genetic regulation involves the binding of cofactors/coenzymes onto a protein in order to make it functional?

A Translational control

B Control of Transcription

C Post Translational Control

D Changing the DNA sequence

E None of the above

Question #37

Which of the following levels of genetic regulation are the most drastic and difficult to reverse?

A Control of Transcription

B Translational control

C Post Translational Control

D Changing the DNA sequence

E None of the above

Question #38

A regulatory protein binding to a regulatory DNA sequence is an example of which of the following levels of gene expression?

A Translational control

B Control of Transcription

C Post Translational Control

D Changing the DNA sequence

E None of the above

Try The Exam

Fresh Grad Lands Job as Real Estate Agent With Help from Professional Writers

Need help with your exam preparation?

Below are the questions for the exam with the choices of answers:

Christian S.

Tyra

Linda

Manush

We accept payment