Exam 3

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Question #1

The restriction enzyme EcoRI creates sticky ends after cutting.

A FALSE

B TRUE

Question #2

A frameshift mutation will not occur if only one nucleotide is added/deleted to the DNA sequence.

A TRUE

B FALSE

Question #3

DNA is positive which allows it to travel down a gel in gel electrophoresis when a current is applied.

A TRUE

B FALSE

Question #4

Heat shock and electroporation are ways to force transformation.

A TRUE

B FALSE

Question #5

Transduction is limited by the DNA carrying capacity of the viral capsid.

A FALSE

B TRUE

Question #6

RecA is a DNA repair enzyme that facilitates the recombination of DNA.

A TRUE

B FALSE

Question #7

Biological fitness has to do with how strong an organism is versus those in its environment.

A TRUE

B FALSE

Question #8

MutL is an enzyme that links MutH and MutS in methyl mismatch repair.

A TRUE

B FALSE

Question #9

The protein that lacZ transcribes is responsible for digesting lactose into glucose and galactose.

A FALSE

B TRUE

Question #10

A bacterial cell’s genome is continuously expressed because it requires all of the proteins it encodes for at all times.

A FALSE

B TRUE

Question #11

Which of the following techniques would I use in order to analyze and visualize the presence of target proteins in a cell/tissue?

A DNA Hybridization

B Immunofluorescence

C DNA sequencing

D PCR

E None of the above

Question #12

Which of the following techniques would I use in order to analyze and visualize the presence of a target sequence of DNA in a cell/tissue?

A PCR

B Immunofluorescence

C DNA Hybridization

D DNA sequencing

E None of the above

Question #13

Which of the following is necessary in a gel electrophoresis in order to determine the size of all of the DNA fragments?

A Ruler

B Positive control

C Negative control

D Loading dye

E DNA ladder

Question #14

Which of the following restriction enzymes produce blunt ends?

A HindIII

B BamHI

C EcoRI

D HaeIII

E None of the above

Question #15

The link between Wolbachia and nematodes can be defined by:

A Predator/prey relationship

B Parasitism

C Endosymbiosis

D Environmental competition

E None of the above

Question #16

Which of the following is a mechanism of evolution?

A Natural selection

B Random mutation

C Reductive evolution

D Genetic drift

E All of the above

Question #17

Transposition is a crucial gene transfer mechanism. However it can also result in which of the following?

A Nonsense mutation

B Increased competency

C Frameshift mutation

D Cell lysis

E None of the above

Question #18

Single-strand recombination produces which of the following after replication and cell division?

A One daughter cell with a recombinant genome and one with a normal genome

B Two daughter cells with normal genomes

C Two daughter cells with recombinant genomes

D The cell would fail to replicate

E None of the above

Question #19

Which of the following horizontal gene transfer mechanisms involve the transfer of genes from a plasmid onto a bacterial host genome?

A Transduction

B Transformation

C Conjugation

D Transposition

E All of the above

Question #20

A prokaryote protects its genomic DNA by doing which of the following?

A Keeping the DNA away from restriction enzymes

B Creating a nucleus

C Adding phosphate groups to sites on the DNA

D Adding methyl groups to sites on the DNA

E None of the above

Question #21

Conjugation involves the transfer of the _____ of the plasmid DNA strand. ______ cell(s) end up with circularized plasmid.

A 3’ end ; The donor

B 5’ end ; The recipient

C 5’ end ; Both

D 3’ end ; The recipient

E 3’ end ; Both

Question #22

Which of the following horizontal gene transfer mechanisms require cell to cell contact to initiate?

A Conjugation

B Transposition

C Transformation

D Transduction

E All of the above

Question #23

Which of the following horizontal gene transfer mechanisms involve the use of bacteriophages?

A Transformation

B Transduction

C Conjugation

D Transposition

E All of the above

Question #24

A heritable mutation has to have which of the following characteristics?

A The mutation is not repaired before replication and the mutation must not alter the amino acid sequence

B The mutation is repaired before replication and the mutation must not alter the amino acid sequence

C The mutation is not repaired before replication and the base sequence must change

D The mutation is repaired before replication and the base sequence must change

Question #25

In methyl mismatch repair, which of the following enzymes identify the nearest methylation site?

A Mut L

B DNA Polymerase III

C Mut H

D Exonuclease

E Mut S

Question #26

In methyl mismatch repair, which of the following enzymes identify the site of the mutation?

A Mut L

B Exonuclease

C Mut H

D DNA Polymerase III

E Mut S

Question #27

Which of the following enzymes are involved in cleaving and removing the phosphodiester backbone in base excision repair?

A DNA glycosylase and DNA ligase

B DNA polymerase I and DNA ligase

C Endonuclease and DNA ligase

D DNA polymerase I and Endonuclease

Question #28

Which of the following enzymes extract the damaged base in base excision repair?

A Endonuclease

B DNA glycosylase

C DNA polymerase I

D DNA ligase

E None of the above

Question #29

You observe a mutation in a gene in which the amino acid sequence now has an early STOP codon. This is known as which of the following?

A Inversion

B Nonsense mutation

C Silent mutation

D Insertion

E Missense mutation

Question #30

You observe a mutation in a gene in which the amino acid sequence is not affected. This is known as which of the following?

A Inversion

B Nonsense mutation

C Missense mutation

D Insertion

E Silent mutation

Question #31

A repressor protein binds to which of the following sequences?

A Operator

B Coding region

C Promoter

D Inducer

E None of the above

Question #32

You observe a lac operon which has a faulty lacI gene that translates to an inactive protein. Which of the following would occur as a result:

A An inability to digest lactose into glucose and galactose

B Constant expression of the lac operon genes

C Constant repression of the lac operon genes

D An inability to transport lactose into the cell

E None of the above

Question #33

In the lactose operon which of the following is responsible for transporting lactose into the cell?

A B-Galactosidase

B Lactose permease

C Allolactose

D Galactose

E LacI

Question #34

In the lactose operon which of the following causes the repressor protein to fall off when it binds to it?

A Lactose permease

B LacI

C Galactose

D Allolactose

E B-Galactosidase

Question #35

The DtxR protein requires the binding of _______ which then _________ the expression of the dtx toxin.

A Fe2+ ; Down-regulates

B H+ ; Down-regulates

C Cl- ; Up-regulates

D Fe2+ ; Up-regulates

E H+ ; Up-regulates

Question #36

Which of the following levels of genetic regulation involves the binding of cofactors/coenzymes onto a protein in order to make it functional?

A Control of Transcription

B Changing the DNA sequence

C Translational control

D Post Translational Control

E None of the above

Question #37

Which of the following levels of genetic regulation are the most drastic and difficult to reverse?

A Changing the DNA sequence

B Translational control

C Post Translational Control

D Control of Transcription

E None of the above

Question #38

A regulatory protein binding to a regulatory DNA sequence is an example of which of the following levels of gene expression?

A Changing the DNA sequence

B Post Translational Control

C Translational control

D Control of Transcription

E None of the above

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