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Fresh Grad Lands Job as Real Estate Agent With Help from Professional Writers

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L7 Working with Proteins

Navigation   » List of Schools  »  West Los Angeles College  »  Chemistry  »  Chem 221 – Biochemistry for Science Majors  »  Fall 2021  »  L7 Working with Proteins

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Below are the questions for the exam with the choices of answers:

Question #1
Dialysis can be used instead of gel filtration chromatography as a desalting device.
A  FALSE
B  TRUE
Question #2
Separation of proteins by gel filtration column chromatography take advantage of differences in
A  The solubility of proteins
B  Isoelectric points of proteins
C  The size of proteins
D  The net charge of the protein
E  all of the above
Question #3
When separating proteins using gel filtration column chromatography, the first proteins to be eluted will be ________________.
A  negatively charged
B  largest
C  more spherical in shape
D  positively charged
E  smallest
Question #4
A distracted student lets a SDS PAGE gel run for too long. When the student realizes her mistake, it is too late and only four out of the five bands that she is supposed to see on the gel appear. Out of the five proteins listed below, which one has probably run out of the gel?
A  ribonuclease A            Mr =   13,700
B  RNA polymerase        Mr = 450,000
C  immunoglobulin G     Mr = 145,000
D  serum albumin            Mr =   68,500
E  cytochrome c            Mr =   13,000
Question #5
Salting out process involves
A  Precipitation of proteins using sodium chloride
B  Precipitation of proteins using copper sulfate
C  Precipitation of proteins using ammonium sulfate
D  None of these
Question #6
Sodium dodecyl sulfate (SDS) gel electrophoresis is often used for
A  determination of protein sedimentation constants
B  protein molecular mass determinations
C  protein purification
D  none of the above
E  all of the above
Question #7
Which of the following amino acids would be first to elute at pH 7 from an anion-exchange column?
A  glutamic acid
B  alanine
C  lysine
D  asparagine
Question #8
What is the purpose of the gel in SDS-PAGE?
A  Acts as a weak ion exchanger
B  It serves as an electrical conductor
C  It serves as a means to physical separate the proteins based on size
D  It serves to reduce the proteins onto their denatured form
Question #9
In a crude extract, globular proteins are usually NOT dissolved in the buffer used for extraction and can be collected by centrifugation.
A  FALSE
B  TRUE
Question #10
The basis of the technique of chromatography for separating components of a mixture is
A  the deflection of charged particles in a magnetic field.
B  the absorption of infrared radiation by the components.
C  the interaction of the components with both stationary and mobile phases.
D  the differing movement of particles of different mass in an electric field.
Question #11
The molecular weight of an unspecified protein, at physiological conditions, is 70,000 Dalton. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of the reducing agent, β-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton.
From these data, describe the native protein in terms of the number of subunits present, their molecular weight, stoichiometry of subunits, and the kinds of bonding existing between the subunits.
A  3 – 30,000 and 20,000 – 1×30,000 and 2×20,000 – disulfide
B  2 – 30,000 and 40,000 – 1×30,000 and 1×40,000 – disulfide
C  2 – 30,000 and 40,000 – 1×30,000 and 1×40,000 – noncovalent
D  4 – 30,000 and 20,000 – 2×30,000 and 2×20,000 – disulfide
Question #12
A characteristic feature of any form of chromatography is the …
A  calculation of an Rf value for the molecules separated.
B  use of a mobile and a stationary phase.
C  use of molecules that are soluble in water.
D  use of an inert carrier gas.
Question #13
The pH at which a protein carries a net zero charge is termed which of the following?
A  K
B  pKa
C  pI
D  pKb
Question #14
The process of passing a mobile phase through a chromatography column is called which one of the following?
A  Washing
B  Partitioning
C  Flushing
D  Elution
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“Kudos to the team for a job well done.  I am sincerely appreciative of the time and effort you gave on my resume.  You did not only help me land the job I had always been dreaming of but you also made me realize how important adding those specific keywords to my resume!  Cheers!

Manush
Fresh Graduate
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