Post Quiz Journal Article 1

A  ABCB6 is expressed in plasma membranes of erythrocytes.

B  ABCB6 colocalizes with markers for the endolysosomal compartment.

C  Silencing of ABCB6 using shRNA interfered with the ability of K562 cells to undergo differentiation.

D  ABCB6 is glycosylated, and glycosylated proteins are very rarely found in mitochondria.

A  Localization of ABCB6 to a specific region of the RBC shows it is important in heme biosynthesis.

B  Knowing the location of ABCB6 in the cell would show the location of all the other ABC family members.

C  Understanding where the ABCB6 transporter is located leads to answering the question of what its function is.

D  The location proves that ABCB6 plays a role in transporting solutes across mitochondrial membranes.

A  TRUE

B  FALSE

A  ABCB6 protein expression is not affected by erythroid differentiation.

B  It upregulates ABCB6 protein expression.

C  It downregulates ABCB6 protein expression.

D  ABCB6 expression is correlated with PPIX fluorescence.

A  It colocalizes with ABCB6 and increases with increasing expression of ABCB6.

B  Its expression is upregulated during erythroid differentiation.

C  It is not influenced by erythroid differentiation and is used as a loading control for the western blots.

D  It is used as to measure increased expression of PPIX-fluorescence.

A  TRUE

B  FALSE

A  Provide evidence that overexpression of HA-tagged ABCB6 in K562 cells was not identified using the ABCB6 antibodies and show that porin was not located in the membranes of red blood cell ghosts.

B  Test four different ABCB6 antibodies; some of which were commerically made and others custom made and show that porin was not located in the membranes of red blood cell ghosts.

C  Show that treatment of RBC and K562 cells overexpressing ABCB6 with PNGase F proved ABCB6 is expressed as a N-glycosylated protein.

D  Provide evidence that overexpression of HA-tagged ABCB6 in K562 cells was not identified using the ABCB6 antibodies and test four different ABCB6 antibodies; some of which were commerically made and others custom made.

A  FALSE

B  TRUE

A  FALSE

B  TRUE

A  Flotillin

B  Alix

C  Tfr

D  Spectrin

E  Tsg 101

A  The level of hemoglobin decreased, as did the amount of ABCB6.

B  The level of hemoglobin remained the same as the control and did not correlate with the increased amount of ABCB6.

C  The increased level of hemoglobin correlated to a decrease in ABCB6 protein.

D  The level of hemoglobin increased as did the amount of ABCB6.

A  Differential centrifugation

B  Exosome Isolation

C  Flow cytometry

D  Confocal Microscopy