Lecture Exam 2 (Ch 5, 6, 7, 8)

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Question #1

Choose the statement that best describes the role of restriction enzymes in genetic engineering.

A Restriction enzymesare used to join different segments of DNA.

B Restriction enzymes are used to purify DNA.

C Restrictionenzymes are used to cut DNA at specific locations.

D Restrictionenzymes are used to sequence long stretches of DNA.

E Restrictionenzymes are used to replicate specific sequences of DNA.

Question #2

DNA replication proceeds in one direction around the bacterial chromosome.

A False

B True

Question #3

When bacteriophages invade bacterial cells, they leave behind DNA that is recognized by the bacteria as foreign and can be eliminated in the event of another attack, reminiscent of adaptive immunity. This natural process can be adapted in the lab so that a combination of guide DNA and nucleases facilitate the cutting of DNA at a precise location in any organism. Here we are describing ________.

A CRISPR

B recombinant DNA technology

C metagenomics

D synthetic biology

E gene therapy

Question #4

Each nucleotide is composed of ________.

A one phosphate, two nitrogenous bases, and one sugar

B two phosphates, one nitrogenous base, and two sugars

C one phosphate, one nitrogenous base, and one sugar

D two phosphates, one nitrogenous base, and one sugar

E one phosphate, one nitrogenous base, and two sugars

Question #5

The expression of genetic traits is the _______.

A genotype

B proteotype

C genome

D proteome

E phenotype

Question #6

Reverse transcriptase is used to make cDNA from an RNA template.

A False

B True

Question #7

Repressible operons are normally turned on in the cell.

A False

B True

Question #8

Synthetic biology is an emerging field of science whereby researchers are creating new molecules and organisms from an array of chemicals, rather than as a result of cellular reproduction.

A True

B False

Question #9

The lagging strand of DNA is made in small segments called Okazaki fragments, each one requiring a primer to begin synthesis, whereas the leading strand, synthesized continuously, does not require a primer to initiate the daughter strand.

A False

B True

Question #10

Induced mutations result from errors in DNA replication.

A True

B False

Question #11

Select the statement that best describes the purpose of gel electrophoresis.

A Gelelectrophoresis separates DNA fragments based on size.

B Gelelectrophoresis enables the amplification of specific DNA sequences.

C Gelelectrophoresis identifies specific segments of DNA.

D Gelelectrophoresis is able to cut DNA in very specific locations.

E Gelelectrophoresis joins different segments of DNA.

Question #12

When DNA is heated to 94 oC, the two strands will separate.

A False

B True

Question #13

Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.

A All of the choices are correct

B several to many chromosomes

C histone proteins

D elongated, not circular, chromosomes

E chromosomes in a nucleus

Question #14

The specific sequence of three bases in mRNA that encodes a particular amino acid is the _______ whereas the complementary sequence of three bases in tRNA that binds to the mRNA is best known as the _______.

A anticodon; codon

B codon; anticodon

C codon; DNA triplet

D gene; complement

Question #15

The emerging field of synthetic biology, while offering promising breakthroughs in medicine and energy production, is not without concern. Apprehension by ethicists is understandable since ________.

A oil and gas prices will increase as a result of the decreased demand for fossil fuels

B mutations in manufactured cells will cause problems for existing organisms

C medical insurance costs will likely skyrocket

D the ability to create life from scratch raises a multitude of issues

Question #16

Transcription occurs in the nucleus or at the nucleoid.

A False

B True

Question #17

Mastery of synthetic biology will allow humans to ________.

A make new organisms using only chemicals as the starting materials

B synthesize chemicals in cells that are missing due to a genetic anomaly

C construct complex molecules in situ that will target cancerous cells in tissues

D design alternative forms of energy production

E All of the choices are possibilities from synthetic biology

Question #18

The _______ strand is synthesized continuously while the ________ strand is synthesized in a series of short fragments called Okazaki fragments.

A lagging; leading

B primary; secondary

C original; primary

D leading; lagging

Question #19

In addition to electrons, which of the following can also be transferred, exchanging energy in the process?

A Glucose

B Carbon dioxide

C ADP

D Carbon

E Hydrogen ion (proton)

Question #20

During which phase of cellular respiration is the majority of ATP formed?

A Processing of pyruvic acid for the Krebs cycle

B Glycolysis

C Electron transport

D Krebs cycle

E All phasesproduce the same number of ATP molecules.

Question #21

Reduction of nitrogen-oxygen ions, such as nitrate, by some bacteria is called _______.

A nitrification

B deamination

C aerobic respiration

D fermentation

E denitrification

Question #22

Fatty acids can be converted to acetyl-CoA for entering the Krebs cycle by the process of _______.

B gluconeogenesis

C beta oxidation

D amination

E deamination

F phosphorylation

Question #23

The property of organisms to integrate catabolic and anabolic pathways to improve cell efficiency is called _______.

A anabolism

B catabolism

C amphibolism

D biosynthesis

E metabolism

Question #24

Each of the following is true of enzymes except ________.

A their active site is specific to the substrate

B All of the choices are true of enzymes.

C they may or may not require cofactors

D they can be used over and over

E they increase the initial energy required for a reaction to start

Question #25

When glucose is broken down by glycolysis during bacterial fermentation, how many ATP are generated?

A 2 ATP

B 38 ATP

C 24 ATP

D 36 ATP

E 3 ATP

Question #26

Enzymes that hydrolyze a peptide bond are called _______.

A peptidases

B aminotransferases

C ligases

D oxidoreductases

E phosphotransferases

Question #27

In bacterial cells, when glucose is completely oxidized by all the pathways of aerobic cellular respiration, what is the maximum number of ATP generated?

A 3 ATP

B 24 ATP

C 38 ATP

D 2 ATP

E 42 ATP

Question #28

Which of the following is not involved in the step that occurs between glycolysis and the Krebs cycle?

A CO2 is released

B Pyruvic acid accepts electrons from NADH

C NADH is formed

D Coenzyme A attaches to an acetyl group

Question #29

Which of the following is not true of anaerobic respiration?

A Involves glycolysis

B Generates some ATP

C Uses the same final electron acceptor as aerobic respiration

D Utilizes an electron transport system

Question #30

How many carbon dioxide molecules are produced by the complete aerobic breakdown of one glucose molecule?

A 5

B 2

C 6

D 3

E 4

Question #31

In a bacterial cell, the genes that code for enzymes that catalyze the hydrolysis of an unusual carbohydrate source would be turned off unless that carbohydrate was the only fuel source available in the media, in which case________.

A the enzymes would be denatured

B the genes coding for the hydrolytic enzymes would be induced

C the genes coding for the hydrolytic enzymes would be constitutive

D the genes coding for the hydrolytic enzymes would be repressed

Question #32

The cell’s metabolic reactions involve the participation of ________ that lower the activation energy needed for the initiation of a reaction.

A coenzymes

B cofactors

C enzymes

D ATP

E vitamins

Question #33

Exergonic reactions ________.

A release energy

B do not occur in anaerobic cellular respiration

C include synthesis of large carbohydrates

D occur when ADP binds to inorganic phosphate to form ATP

E only occur in heterotrophs

Question #34

FAD, NAD, and coenzyme A are all _______ carriers.

A None of the choices are correct.

B electron

C hydrogen andelectron

D ATP

E hydrogen

Question #35

Each of the following are denaturing agents except ________.

A high pH

B low temperature

C low pH

D high temperature

E All of the choices are correct.

Question #36

An organism that cannot tolerate an oxygen environment is a(n) _______.

A obligate aerobe

B facultative anaerobe

C microaerophile

D obligate anaerobe

E aerotolerant anaerobe

Question #37

Most of the dry weight of a microbial cell is from _______.

A organic compounds

B water

C minerals

D salts

E inorganic compounds

Question #38

Aerobic respiration is an example of _______.

A photoheterotrophy

B chemoheterotrophy

C photoautotrophy

D methanoheterophy

E photosynthesis

Question #39

Bacterial biofilms stimulate the immune system differently than free (planktonic) bacteria.

A False

B True

Question #40

All of the following could find a location in or on body tissues suitable for growth except _______.

A psychrophiles

B anaerobes

C mesophiles

D capnophiles

E facultative anaerobes

Question #41

What compound is the most abundant in a cell?

A NH 3

B CH 4

C Glucose

D CO 2

E H 2O

Question #42

The term obligate refers to________.

A existing in a very narrow niche

B using light for energy production

C using chemicals for energy production

D the ability to exist in a wide range of conditions

E using oxygen for metabolism

Question #43

The toxic superoxide ion is converted to harmless oxygen by two enzymes, ________.

A catalase and hydrogen peroxidases

B superoxide dismutase and oxidase

C superoxide dismutase and hydrogen peroxidases

D catalase and oxidase

E superoxide dismutase and catalase

Question #44

The movement of molecules from an area of high concentration to an area of lower concentration is called _______.

A facilitated diffusion

B endocytosis

C osmosis

D active transport

E diffusion

Question #45

Human pathogens fall into the group termed _______.

A thermophiles

B mesophiles

C halophiles

D psychrophiles

E acidophiles

Question #46

Facilitated diffusion is limited by________.

A osmotic pressure

B substrate concentration

C the size of the cell

D size of the pores in the membrane

E carrier proteins in the membrane

Question #47

Select all of the cellular transport mechanisms that require energy.

A Facilitated diffusion and Simple diffusion

B Facilitated diffusion and Carrier-mediated active transport

C Simple diffusion and Bulk transport (phagocytosis, pinocytosis)

D Carrier-mediated active transport and Bulk transport (phagocytosis, pinocytosis)

Question #48

An important mineral ion that is a component of chloroplasts and stabilizer of membranes and ribosomes is _______.

A iron

B zinc

C magnesium

D calcium

E potassium

Question #49

Nutrient absorption is mediated by the________.

A peptidoglycan layer

B cell wall

C cell membrane

D proteins in the periplasmic space

E nuclear membrane

Question #50

Which of the following is not a major element of a microbial cell?

A Hydrogen

B Nitrogen

C Oxygen

D Copper

E Carbon

Question #51

What type of media is used to demonstrate oxygen requirements of microbes?

A Thioglycollate

B Blood agar

C Sulfite polymyxin sulfadiazine

D None of the choices are correct.

E Sodium chloride

Question #52

When whole cells or large molecules in solution are engulfed by a cell, this endocytosis is specifically termed _______.

A facilitated transport

B pinocytosis

C facilitated diffusion

D exocytosis

E phagocytosis

Question #53

Lysogeny refers to ________.

A viral genome inserting into bacterial host chromosome

B altering the host range of a virus

C None of the choices are correct

D virion exiting host cell

E latent state of herpes infections

Question #54

Viral nucleic acids include which of the following?

A Double-stranded RNA

B All of the choices are correct

C Single-stranded RNA

D Single-stranded DNA

E Double-stranded DNA

Question #55

Mammalian viruses capable of starting tumors are _____.

A inclusion bodies

B cytopathic

C oncoviruses

D syncytia

E chronic latent viruses

Question #56

Host range is limited by________.

A type of host cell receptors on cell membrane

B size of the host cell

C age of the host cell

D type of nucleic acid in the virus

E All of the choices are correct

Question #57

Which of the following is incorrect about prophages?

A Cause lysis of host cells

B Occur when temperate phages enter host cells

C Present when the virus is in lysogeny

D Formed when viral DNA enters the bacterial chromosome

E Replicated with host DNA and passed on to progeny

Question #58

Infectious protein particles are called _______.

A viroids

B spikes

C prions

D oncogenic viruses

E phages

Question #59

Viruses that infect bacteria are specifically called _______.

A satellite viruses

B bacteriophages

C viroids

D All of thechoices infect bacteria.

E prions

Question #60

Viral tissue specificities are called _____.

A uncoating

B receptacles

C virions

D ranges

E tropisms

Question #61

Viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called _____.

A latent

B oncogenic

C prions

D viroids

E delta agents

Question #62

A negative-sense RNA virus ________.

A must synthesize a negative RNA copy of its genome

B is a special form of tRNA used by viruses

C always codes for RNA polymerase

D is ready for immediate translation

E must synthesize a positive RNA copy of its genome

Question #63

Which of the following is not a typical capsid shape?

A Icosahedron

B Tetrahedral

C Complex

D Helical

E All of thechoices are capsid shapes.

Question #64

The process of dissolving the envelope and capsid to release the viral nucleic acid is _______.

A adsorption

B synthesis

C assembly

D uncoating

E penetration

Question #65

A(n) _______ is the protein shell around the nucleic acid core of a virus.

A capsid

B envelope

C monolayer

D spike

E capsomere

Question #66

Select the three methods that are used to cultivate viruses.

A Use of enriched broth media
Use of cellculture techniques
Use of enrichedagar media

B Inoculation ofembryonated eggs,
Use of animalinoculation,
Use of cellculture techniques

C Use of enriched broth media,
Use of enrichedagar media,
Inoculation ofembryonated eggs

D Use of animalinoculation
Use of enriched broth media
Use of enrichedagar media

Question #67

During lysogeny, an inactive prophage state occurs when the viral DNA is inserted into the ________.

A host nucleus

B host cell membrane

C host DNA

D host cytoplasm

E host nucleolus

Question #68

Select the three choices that are the primary purposes of cultivating viruses.

A To produce virusfor bioweapons use
To produce chemicals such as alcohol and acetone
To isolate viruses from clinical specimens

B To produce virusfor bioweapons use
To prepare viruses for vaccines
To produce chemicals such as alcohol and acetone

C To isolate viruses from clinical specimens
To research the biology of viruses
To prepare viruses for vaccines

D To research the biology of viruses
To produce virusfor bioweapons use
To produce chemicals such as alcohol and acetone

Question #69

The envelope of enveloped viruses________.

A is obtained by viral budding or exocytosis

B is only composed of host endomembrane

C None of the choices are correct

D does not contain spikes

E is identical to the host plasma membrane

Question #70

Persistent viruses that can reactivate periodically are _____.

A oncoviruses

B syncytia

C inclusion bodies

D cytopathic

E chronic latent viruses

Question #71

Which of the following will not support viral cultivation?

A Continuous cell cultures

B Live lab animals

C Embryonated bird eggs

D Primary cell cultures

E All of thechoices will support viral cultivation.

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Lecture Exam 2 (Ch 5, 6, 7, 8)

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