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Fresh Grad Lands Job as Real Estate Agent With Help from Professional Writers

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Module 3 Quiz

Navigation   » List of Schools  »  University of California San Diego  »  Chemistry  »  Chem 40005 – Clinical Chemistry  »  Summer 2021  »  Module 3 Quiz

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Below are the questions for the exam with the choices of answers:

Question #1
You would like to offer a service to the researchers in your institution for identification and quantitative analysis of proteins produced by microorganisms in liquid media using your HPLC-mass spectrometer system. Which one of the ionization techniques is best suited for this analysis?
A  Chemical
B  Electron
C  Electrospray
D  Any of these would work with HPLC-MS.
Question #2
The component of an immunoglobulin molecule that determines the antigenic specificity of that antibody is the
A  epitope.
B  light chain.
C  disulfide bond linkage between the heavy and light chains.
D  sequence at the amino terminal end of the heavy and light chains.
Question #3
In partition chromatography, separation is based on
A  the molecular size of solutes in a solution and the size of the pores on the beads.
B  hydrogen bonding and hydrophobic interactions.
C  the ability of one component immobilized on a stationary phase to capture specific molecules in the mobile phase.
D  the differences in the relative solubility of compounds between the stationary and mobile phases.
Question #4
The most significant improvement in the quality of laboratory test results can be attributed to the ability of automation to
A  reduce error and variability caused by manual manipulation.
B  reduce sample volumes and laboratory involvement.
C  reduce costs.
D  increase work capacity.
Question #5
You are preparing placental tissue for separation and isolation of a specific enzyme. The enzyme has a negative charge, so you want to use chromatography to perform your task. Which one of the following chromatographic separation mechanisms would be most useful?
A  Adsorption
B  Partition
C  Affinity
D  Ion-exchange
Question #6
The type of separation mechanism for chromatography that involves, as one example, use of immunologic principles is _____ chromatography.
A  affinity
B  ion exchange
C  adsorption
D  partition
Question #7
The substance that is typically injected into an animal to elicit an immune response and induce formation of an antibody is the
A  antibody.
B  immunogen.
C  immunochemical label.
D  enzyme.
Question #8
A substance that cannot elicit an immune response alone but that must be conjugated to a carrier molecule to cause antibody production is referred to as a(n)
A  antigen.
B  hapten.
C  conjugate.
D  immunogen.
Question #9
In regard to mass spectrometry, what is a molecular ion?
A  It is the sum of all ions produced displayed as a function of time.
B  It is a component of a compound in solution.
C  It is the unfragmented ion of the original molecule being studied.
D  It is the ion with the highest abundance in the mass spectrum of a compound.
Question #10
Because of the ability to identify and quantify proteins in a complex mixture, mass spectrometry combined with separation methods is an excellent analytical tool used specifically in the field of
A  proteomics.
B  inborn errors of metabolism.
C  trace metal analysis.
D  genomic research.
Question #11
The type of automated analyzer in which the operator is able to use in-house reagents or reagents purchased from suppliers different from the analyzer’s manufacturer is referred to as a(n)
A  random-access analyzer.
B  open analyzer system.
C  closed analyzer system.
D  discrete analyzer.
Question #12
In a mass spectrometer, ion detection is typically accomplished through the use of an electron multiplier. This involves
A  collection of the ion current directly.
B  a densitometer that measures ion effluent and produces a “peak” of activity visible on a computer monitor.
C  four parallel electrically conductive rods arranged in a square array that increases electron number.
D  a chain of dynodes that “multiplies” the number of electrons to provide a detectable signal.
Question #13
Some analytes must be derivatized to increase their column retention or detectability. Derivatization means
A  adding fluorescent labels or combining the analyte with chiral reagents or other chemicals to increase detectability.
B  using multiple detectors to assist in identification.
C  altering the chemical structure of the analyte to increase detection and specificity.
D  removing dissolved gases in the solvent to produce a clear chromatogram.
Question #14
An antibody that has very strong affinity to a single antigenic epitope is referred to as a(n) _____ antibody.
A  multiclonal
B  polyclonal
C  affinity-purified
D  monoclonal
Question #15
In a mass spectrometer, the ion with the highest abundance in the mass spectrum that is assigned a relative abundance of 100% is referred to as the
A  ion trap.
B  time-of-flight ion.
C  ionic chromatogram.
D  base peak.
Question #16
A measure of peak separation in a chromatographic method that equals the difference in retention time for two components divided by the average of their peak widths is the definition of
A  affinity.
B  resolution.
C  derivatization.
D  retention factor.
Question #17
In the thin-layer chromatography procedure for drug screening, the retention factor (Rf) value for a compound is given by the
A  ratio of distance moved by the unknown solute to distance moved by the solvent in the mobile phase.
B  distance moved by the mobile phase front from the point of application compared with a control.
C  measurement in centimeters of the distance the solute moved in the mobile phase from the point of application.
D  rate of movement of the mobile phase through the adsorbent compared with standards and controls.
Question #18
Using whole blood as the specimen of choice in an automated analytical system
A  allows for the avoidance of carry-over.
B  essentially eliminates specimen preparation time.
C  allows the operator to use a secondary tube for analysis.
D  keeps the specimen from undergoing degradation.
Question #19
What is the function of the vacuum system in a mass spectrometer?
A  To detect, identify, and quantify ion mass in a compound
B  To separate the negative ions from the positive ions with a magnetic field
C  To produce an ion from a neutral atom or molecule in the initial step
D  To keep ions from colliding during interactions with the magnetic or electric fields
Question #20
In a precipitin reaction, an excess of antigen over an antibody will lead to
A  all antibody sites bound by antigen with no formation of precipitate.
B  a lack of reaction between an antibody and antigen, and no precipitate.
C  cross-linking of antigen and an antibody with maximal precipitate formation.
D  all antigen sites bound with an antibody and no precipitate formation.
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Linda
Start Up Founder

“Kudos to the team for a job well done.  I am sincerely appreciative of the time and effort you gave on my resume.  You did not only help me land the job I had always been dreaming of but you also made me realize how important adding those specific keywords to my resume!  Cheers!

Manush
Fresh Graduate
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