Exam 3

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Question #1

The restriction enzyme EcoRI creates sticky ends after cutting.

A TRUE

B FALSE

Question #2

A frameshift mutation will not occur if only one nucleotide is added/deleted to the DNA sequence.

A TRUE

B FALSE

Question #3

DNA is positive which allows it to travel down a gel in gel electrophoresis when a current is applied.

A TRUE

B FALSE

Question #4

Heat shock and electroporation are ways to force transformation.

A FALSE

B TRUE

Question #5

Transduction is limited by the DNA carrying capacity of the viral capsid.

A FALSE

B TRUE

Question #6

RecA is a DNA repair enzyme that facilitates the recombination of DNA.

A TRUE

B FALSE

Question #7

Biological fitness has to do with how strong an organism is versus those in its environment.

A FALSE

B TRUE

Question #8

MutL is an enzyme that links MutH and MutS in methyl mismatch repair.

A FALSE

B TRUE

Question #9

The protein that lacZ transcribes is responsible for digesting lactose into glucose and galactose.

A TRUE

B FALSE

Question #10

A bacterial cell’s genome is continuously expressed because it requires all of the proteins it encodes for at all times.

A TRUE

B FALSE

Question #11

Which of the following techniques would I use in order to analyze and visualize the presence of target proteins in a cell/tissue?

A PCR

B Immunofluorescence

C DNA Hybridization

D DNA sequencing

E None of the above

Question #12

Which of the following techniques would I use in order to analyze and visualize the presence of a target sequence of DNA in a cell/tissue?

A PCR

B DNA Hybridization

C DNA sequencing

D Immunofluorescence

E None of the above

Question #13

Which of the following is necessary in a gel electrophoresis in order to determine the size of all of the DNA fragments?

A DNA ladder

B Ruler

C Loading dye

D Negative control

E Positive control

Question #14

Which of the following restriction enzymes produce blunt ends?

A BamHI

B EcoRI

C HindIII

D HaeIII

E None of the above

Question #15

The link between Wolbachia and nematodes can be defined by:

A Endosymbiosis

B Environmental competition

C Parasitism

D Predator/prey relationship

E None of the above

Question #16

Which of the following is a mechanism of evolution?

A Natural selection

B Random mutation

C Genetic drift

D Reductive evolution

E All of the above

Question #17

Transposition is a crucial gene transfer mechanism. However it can also result in which of the following?

A Nonsense mutation

B Increased competency

C Frameshift mutation

D Cell lysis

E None of the above

Question #18

Single-strand recombination produces which of the following after replication and cell division?

A The cell would fail to replicate

B Two daughter cells with normal genomes

C Two daughter cells with recombinant genomes

D One daughter cell with a recombinant genome and one with a normal genome

E None of the above

Question #19

Which of the following horizontal gene transfer mechanisms involve the transfer of genes from a plasmid onto a bacterial host genome?

A Transduction

B Conjugation

C Transposition

D Transformation

E All of the above

Question #20

A prokaryote protects its genomic DNA by doing which of the following?

A Adding phosphate groups to sites on the DNA

B Creating a nucleus

C Keeping the DNA away from restriction enzymes

D Adding methyl groups to sites on the DNA

E None of the above

Question #21

Conjugation involves the transfer of the _____ of the plasmid DNA strand. ______ cell(s) end up with circularized plasmid.

A 3’ end ; Both

B 3’ end ; The donor

C 3’ end ; The recipient

D 5’ end ; Both

E 5’ end ; The recipient

Question #22

Which of the following horizontal gene transfer mechanisms require cell to cell contact to initiate?

A Transposition

B Transduction

C Transformation

D Conjugation

E All of the above

Question #23

Which of the following horizontal gene transfer mechanisms involve the use of bacteriophages?

A Transduction

B Conjugation

C Transformation

D Transposition

E All of the above

Question #24

A heritable mutation has to have which of the following characteristics?

A The mutation is repaired before replication and the mutation must not alter the amino acid sequence

B The mutation is repaired before replication and the base sequence must change

C The mutation is not repaired before replication and the mutation must not alter the amino acid sequence

D The mutation is not repaired before replication and the base sequence must change

Question #25

In methyl mismatch repair, which of the following enzymes identify the nearest methylation site?

A DNA Polymerase III

B Mut L

C Mut S

D Exonuclease

E Mut H

Question #26

In methyl mismatch repair, which of the following enzymes identify the site of the mutation?

A Mut L

B Mut H

C Mut S

D DNA Polymerase III

E Exonuclease

Question #27

Which of the following enzymes are involved in cleaving and removing the phosphodiester backbone in base excision repair?

A Endonuclease and DNA ligase

B DNA polymerase I and Endonuclease

C DNA glycosylase and DNA ligase

D DNA polymerase I and DNA ligase

Question #28

Which of the following enzymes extract the damaged base in base excision repair?

A Endonuclease

B DNA polymerase I

C DNA glycosylase

D DNA ligase

E None of the above

Question #29

You observe a mutation in a gene in which the amino acid sequence now has an early STOP codon. This is known as which of the following?

A Insertion

B Missense mutation

C Nonsense mutation

D Inversion

E Silent mutation

Question #30

You observe a mutation in a gene in which the amino acid sequence is not affected. This is known as which of the following?

A Nonsense mutation

B Inversion

C Insertion

D Silent mutation

E Missense mutation

Question #31

A repressor protein binds to which of the following sequences?

A Promoter

B Inducer

C Operator

D Coding region

E None of the above

Question #32

You observe a lac operon which has a faulty lacI gene that translates to an inactive protein. Which of the following would occur as a result:

A Constant expression of the lac operon genes

B An inability to digest lactose into glucose and galactose

C An inability to transport lactose into the cell

D Constant repression of the lac operon genes

E None of the above

Question #33

In the lactose operon which of the following is responsible for transporting lactose into the cell?

A B-Galactosidase

B Lactose permease

C LacI

D Galactose

E Allolactose

Question #34

In the lactose operon which of the following causes the repressor protein to fall off when it binds to it?

A Allolactose

B LacI

C Lactose permease

D Galactose

E B-Galactosidase

Question #35

The DtxR protein requires the binding of _______ which then _________ the expression of the dtx toxin.

A H+ ; Up-regulates

B Cl- ; Up-regulates

C Fe2+ ; Up-regulates

D Fe2+ ; Down-regulates

E H+ ; Down-regulates

Question #36

Which of the following levels of genetic regulation involves the binding of cofactors/coenzymes onto a protein in order to make it functional?

A Control of Transcription

B Post Translational Control

C Translational control

D Changing the DNA sequence

E None of the above

Question #37

Which of the following levels of genetic regulation are the most drastic and difficult to reverse?

A Control of Transcription

B Changing the DNA sequence

C Post Translational Control

D Translational control

E None of the above

Question #38

A regulatory protein binding to a regulatory DNA sequence is an example of which of the following levels of gene expression?

A Post Translational Control

B Changing the DNA sequence

C Translational control

D Control of Transcription

E None of the above

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